Xenbase Image ID: 136010

FIG. 2. Localization of c-myc RNA in the limb regenerate. In situ hybridizations were carried out as described in the text. (A and B) Sections were hybridized with a 35S-labeled mb. antisense c-myc RNA probe. (A) Regenerate under dark-field illumination. A high level of labeling was intermediate layers of the epithelium (star). The signal was much lower in the outermost layers (arrows). A moderate labeling was also detected in the mesenchymal cells (mb). (x 160.) (B) The same regenerate under bright-field illumination. The rim of autoradiographic grains lining the tissue sections is an artifact created during the procedure of autoradiography (drying of the emulsion). (x160.) (C and D) Control sections of the regenerate hybridized with a labeled sense c-myc RNA probe. (C) Dark-field illumination. Labeling is not above background in the epithelum (ep) and in the mesenchyme of the outgrowth (mb). (x200.) (D) Bright-field illumination. (x200.) Image published in: Géraudie J et al. (1990) Copyright © 1990. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue myc.S c-myc, cmyc, myc-a, myc-b, X-cmyc, Xcmyc X. laevis Sometime during NF stage 53 to NF stage 56 epidermis regenerating forelimb wound epidermis wound epidermis inner layer wound epidermis outer layer distal mesenchyme Image source: Published Larger Image Printer Friendly View

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