Xenbase Image ID: 136034

Figure 3. An early Fgf signal is required for development of the ventronasal retina. A, B: DAPI-labeled transverse cryosections of the eyes of Stage 40 Xenopus laevis embryos incubated in a control DMSO solution (A), or 150–200 μM solution of SU5402 (B) for 5 hr beginning at Stage 12. The dorsoventral axis, optic nerve head (white arrowheads), inner plexiform layer (ipl) and outer plexiform layer (opl) (red arrows) of the eye are indicated. C: The distance from the optic nerve head to the lateral edge of the dorsal and ventral retina was measured in control and inhibitor treated embryos using the ipl as a guide. D, E: The angle between the anteroposterior (A/P) axis and a line bisecting the eye and contacting either the central edge of the ventrotemporal (θ, D) or ventronasal (θ1, E) retina was measured in those Fgfr inhibitor-treated embryos that exhibited coloboma. F: These angles were compared to the angle between the A/P axis and a line bisecting the eye through the choroid fissure (cf) in control embryos. Values in graphs are mean ± SEM, and average values were compared where indicated using unpaired t-tests. *P ≤ 0.05, **P < 0.01.Download figure to PowerPoint Image published in: Atkinson-Leadbeater K et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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