XB-IMG-136055
Xenbase Image ID: 136055
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Figure 5. XMLC2 expression during development. Total RNA
from embryos, cultured explants, and tissues was tested for
XMLC2 mRNA by use of an RNase protection assay. (Lane 1)
tRNA control; (lanes 2-10) two embryos from neurula to tadpole
stages of development (stages 18-35, as indicated); (lanes
11,12) five anterior-dorsal (D) and anterior-ventral (V) regions
of swimming tadpoles (stage 37); (lanes 13,14) the equivalent of
five blastula animal pole explants treated with 32 or 80 U/ml of
activin A and cultured until stage 42; (lanes 15-17) two tadpole
(stage 42) heart tubes (EH), 0.5 txg of adult heart (H), and 10 ~g
of adult skeletal muscle (Sk). As an internal control, a probe for
EF1 a mRNA was included in each assay. An end-labeled HinfI
digest of pBR322 was used for approximate size markers. Image published in: Chambers AE et al. (1994) Copyright © 1994. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |