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Figure 7. Ectopic expression of SL1 activates XMLC2 gene transcription in animal pole explants. Cultured animal pole explants from injected embryos were analyzed by RNase protection at the neurula stage for expression of the heart-specific XMLC2 gene. Levels of EFla mRNA were monitored in the same assay. (P) Undigested probe; (lane 1) 10 ~g of tRNA; (lane 2) control explants (uninjected); (lanes 3-5) explants from embryos injected with SL1 and SL2 RNA, individually or in combination (as indicated); (lanes 6, 7) 5 ~tg of late neurula (stage 20) and tadpole (stage 37) RNA, respectively. (Size markers as in earlier figures).

Image published in: Chambers AE et al. (1994)

Copyright © 1994. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.

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