Xenbase Image ID: 136081

Figure 7. Xnot expression is altered by cycloheximide and injected Xwnt-8, DVR- 4, and FGF dominant-negative receptor RNAs. Xnot expression was analyzed by the whole-mount in situ procedure using pigmented embryos. (AI Cycloheximide was added to stage 9 embryos that were then left to develop until control embryos reached stage 12. Lateral view with the animal pole at top. The lack of staining in the vegetal region is an artifact of the whole-mount procedure. (B} Cycloheximide was added to stage 10 embryos that were then left to develop until control embryos reached stage 12. Vegetal view with dorsal side at top. (C) Embryos were injected with Xwnt-8 RNA in one of the ventral vegetal cells of an 8- to 16-cell embryo. Embryos were fixed at stage 11. Vegetal view. Note the two sites of Xnot expression at the equator (arrows). (D) Embryos were injected with DVR-4 RNA at the equator in the two dorsal cells of four-cell embryos. Embryos were fixed at the equivalent of stage 11. Vegetal view with dorsal side at top. Note the lack of Xnot expression. (E) Embryos were injected with RNA encoding the dominant-negative mutant of the Xenopus FGF receptor in the two dorsal cells of four-cell embryos. Embryos were fixed at the equivalent of stage 12. Vegetal view with dorsal side at top. No Xnot expression was detected. Image published in: von Dassow G et al. (1993) Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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