Xenbase Image ID: 136086

Figure 3. Expression of molecular markers at neural plate and gastrula stages in ASH3b RNA-injected embryos. (A-D, F, G, I, J) The dorsal side of stage 13.5-14 embryos. Anterior is at the top. (A,F) Schematic diagrams showing the expression patterns of N-CAM, twist, hairy2a, and EpA molecular markers in a wild-type embryo (A) and an injected embryo (F) (see text for references). (B) CNS tissue, identified by N-CAM mRNA expression, is expanded on the injected side (inj). The arrows show the lateral limits of N-CAM expression; the position of the midline is marked. (C) twist mRNA is present in the prospective neural crest on the control side but not on the injected side (arrows). Midline staining in C is twist expression in the notochord and head mesoderm (unaffected by ASH3b). The dorsal limit of expression of the epidermal antigen EpA (brown staining) is moved away from the midline after ASH3b RNA injection (D). (E) The anterior end of a different EpA-stained embryo. In D, E, and G the injected side is marked by nuclear 13-galactosidase staining (blue dots). (G) hairy2a mRNA expression at the border of the neural plate is broader, at a lower level, and farther laterally from the midline on the injected side (compare location of arrows), but midline expression appears unaffected. (H) Dorsal view of a gastrula embryo (stage 11.5) showing the expansion of the area surrounded by hairy2a (purple staining) on the injected side (arrows). The injected side is marked by red staining for nuclear ~-galactosidase. The position of the blastopore lip is shown (white arrows). (I) Expression of Xotch mRNA is elevated on the ASH3b-injected side and wider (compare distance of arrows from the unstained midline). The trigeminal placode (t) is separate from the neural plate on the uninjected control side but contained within the expanded Xotch staining on the injected side. (l) myoDb mRNA is expressed at normal levels on both sides of this injected embryo, but the expressing cells do not extend as far anteriorly and spread farther laterally on the injected side. Image published in: Turner DL and Weintraub H (1994) Copyright © 1994. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

Return to previous page