XB-IMG-136122
Xenbase Image ID: 136122
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Figure 5. Induction of Xlim-l expression in animal explants. [A] Stage 8-8.5 animal explants were cultured in the presence of different
factors for 4 hr. (B) Stage 8-8.5 explants were cultured for 5 hr until siblings reached stage 10.5. (C) Stage 9-9.5 animal explants were
cultured for 30 min in CHX, 30 min in CHX plus activin A, and 2 hr in activin A; siblings reached stage 10-10.5. Five micrograms of
total RNA from each sample was analyzed for expression of different genes, as indicated. In A, hybridization with EF-la, and in B and
C, staining with ethidium bromide (not shown) verified that closely similar levels of RNA had been loaded. The following factor
concentrations were used: activin A, 50 pM (1.4 ng/ml); RA, 10 JJLM; TGF-p2, 200 ng/ml; TGF-p3, 50 ng/ml; bFGF, 100 ng/ml;
cycloheximide (CHX), 5 |xg/ml. Image published in: Taira M et al. (1992) Copyright © 1992. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |