Xenbase Image ID: 136148

Fig. 6. Loss of ADMP2 and heart formation. (A and B) Injection of 10 ng of an antisense ADMP2 morpholino oligonucleotide into each of the two anterior blastomeres of a 4-cell stage embryo inhibited the expression of the heart marker Nkx2.5 (c-MO: control morpholino; A-MO: antisense ADMP2 morpholino). (C) This effect was reversed by co-injection of 5 pg of ADMP2 δ-5′ UTR RNA (total 10 pg) (black arrowheads). (D) Transverse sections of c-MO-injected embryos showed that Nkx2.5 was expressed in a tube of myocardium (white arrowheads) as well as the pericardial roof (black arrowheads in panel D). (E) A-MO-injected embryos, on the other hand, showed only faint expression of Nkx2.5 (white arrowhead in panel E), and the heart tube was not formed. (F) Rescue of the A-MO phenotype, as in panel C. (G, H) Anterior injection of 10 ng A-MO also reduced the expression of TnIC, another heart marker (black arrowhead in panel K compared to that in panel J) (82%; Table 1). Image published in: Kumano G et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 32 heart heart primordium myocardium Image source: Published Larger Image Printer Friendly View

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