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Figure 4. Transcriptional Activity and Binding Selectivity of Chordate RARsTranscriptional activity is shown in (A–D) and (I–L), and corresponding binding selectivity in (E–H) and (M–P). Identities of the chordate RARs for each activity-selectivity pair are indicated above each bar graph. Transcriptional activity is shown in (A–D) for LampRAR, AmphiRAR, PmRAR, and AncRAR, and that of AmphiRAR mutants is shown in (I–L). Partial proteolysis maps of the different in vitro-translated RARs are shown in (E–H) and (M–P). Chimeric GAL-RAR(LBD) transactivation methods, colour code of the transactivation figures, and contents of each proteolysis gel are as in Figure 3. Protected bands in the presence of BMS641 are indicated by an asterisk, and slightly protected bands are indicated by arrowheads. Image published in: Escriva H et al. (2006) Copyright: © 2006 Escriva et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |