Xenbase Image ID: 136200

Figure 2. Expression variation among the samples. (A) and (B) show scatter plot comparisons of the mean Nog+Dkk or Dor stg. 10 log2 expression values vs the mean stg. 10 Ven log2expression values. Probe sets measuring two known organizer genes, otx2 (green) and gsc (red), are labeled within the plots (otx2 probe sets: Xl.1268.1.S1_at, Xl.3004.1.A1_at, Xl.11672.1.A1_at, and XlAffx.1.11.S1_at; gsc probe sets: Xl.801.1.A1_at, Xl.801.1.S1_at, and Xl.801.1.S1_s_at). (C) R-square regression value summaries for each experimental condition compared to the stage-matched ventral condition. (D) Scatter plot comparison of the mean Nog and Ven clutch-1 log2 expression values vs. the mean Nog and Ven clutch-2 log2 expression values. Note that the clutch variation clearly exceeds differences between the experimental conditions (A-C). Contributing to this variation, many genes show a shift in the average expression between the two clutches, but retain a similar pattern in response to the experimental conditions. As an example, log2 expression values for otx5-A (Xl.3452.1.A1_at) are shown in (E). After standardizing the average log2 expression of both clutches, on a gene-by-gene basis, the comparison from (D) was repeated in (F), eliminating most clutch variation. Image published in: Hufton AL et al. (2006) Copyright © 2006 Hufton et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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