Xenbase Image ID: 136235

Figure 8. Semi-quantitative analysis of the localization of endogenous mRNAs. To estimate the extent of localization of each endogenous mRNA, images were collected from 30–40 cells using identical laser settings from the same experiment shown in Figure 7. All raw images were assembled into a montage and a threshold was applied to help identify mRNA labelled in distal processes. A cell was considered to be positive for localization if mRNA could be detected in a process greater than 40 μm away from the cell body. If no signal could be detected greater than 10 μm away from the cell body the cell was considered to be negative for mRNA localization. About 15–30 percent of all cells showed some signal in processes 10–40 μm away from the cell body. These cells were excluded from the graph since they added little information to this analysis. Image published in: Andken BB et al. (2007) Copyright © 2007 Andken et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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