Xenbase Image ID: 136256

Figure 3. Homology modeling, network predictions, and experimental validation suggest a trafficking function for Fuz. (a, b) Rendered protein models (Open-Source PyMOL 0.99rc6 software). (a) Sedl N-terminal domain (pdb:1H3Q). (b) 1H3Q based homology threaded model of the C- terminal -aa(s) 287–419 - of Xenopus Fuz protein. (c) Illustration of experimentally-derived protein-protein interactions (see Supplemental Methods) linking Dvl2 with longin-domain proteins AP1s, AP2s, and SedlP as well as CLAMP. (d) Mosaic imaging of live embryo expressing CLAMP-GFP, which localizes to ciliary axonemes and apical tips. A nucRFP marks the nuclei, serving as a lineage tracer for co-injected Fuz MO. The confocal slice reveals a loss of apical localized CLAMP-GFP at ciliary tips in FUZMO cells (inset shows merge of (e) and a more basal slice to display nucRFP signal). Scale bar in (e) = 10uM. Image published in: Gray RS et al. (2009) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

Return to previous page