Xenbase Image ID: 136257

Figure 4. Fuzzy and RSG1 control trafficking to basal bodies as well as to the tips of cilia. (a, b) Fuz knockdown disrupts localization of CLAMP-GFP to the ciliary rootlet. Confocal stacks of formaldehyde fixed Xenopus epidermis expressing centrin-RFP and CLAMP-GFP mRNAs. (a) Multi-ciliated cell in x-y view from an uninjected control embryo exhibits elongated CLAMP-GFP signal extending from relatively evenly spaced basal bodies (centrin-RFP). (a′) Higher magnification view of the x-y section from [a]. (a″) X-Z projection of the stack shown in [a′] displays apical co-localization of centrin-RFP and CLAMP-GFP. (b) Ciliated cell in an x-y view of the apical surface in a Fuz morphant reveals defects in the spacing of centrin-RFP signal and defects in elongated CLAMP-GFP signal. Additionally CLAMP-GFP signal is not faithfully co-localized with centrin-RFP signal in many cases. (b′) Higher magnification view of the x-y section from [b]. (b″) X-Z-projection of the stack shown in [b′] reveals apical alignment of the centrin-RFP signal however in many cases the CLAMP-GFP signal is below the apical surface in large punctae. (c–e) Mosaic imaging of live agarose embedded embryo. CLAMP-GFP highlights a variety of epidermal structures. RFP-Histone 2B (nuc-RFP) serves as a lineage tracer for morpholino-injected cells. (c, d) 3D projections (x-z). RSG1 MO (+ nucRFP cells) display defects in normal CLAMP-GFP localization along the apical surface. (e) Confocal slice (x-y) exhibiting a loss of apical localized CLAMP-GFP to ciliary tips in RSG1 MO cells (inset shows merge of (e) and more basal slice to display nucRFP signal). Scale bars in (c, d) are 3uM; scale bar in (e) is 10μm. Image published in: Gray RS et al. (2009) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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