XB-IMG-136258
Xenbase Image ID: 136258
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Figure 5. Knockdown of Fuz disrupts exocytosis in mucus-secreting cells. (a) Wild type multi-ciliated cell (left) flanked by secretory cells in Xenopus mucociliary epidermis. Control cells have an average of over 90 open exocytic pits per cell. (b) Fuz morphants display defects in ciliogenesis in multi-ciliated cell (left) and failure of exocytosis in mucus-secreting cells (note the absence of exocytic pits indicated by white arrowheads in [a]). Green arrowheads indicate apical membrane blebs (see also panel F, below). In a representative experiment, Fuz morphant cells had fewer than 5 exocytc pits per cell on average (difference from control is significant by the Mann-Whitney U-test; p<0.0001). Scale bars in A, B = 5μm (c) Mosaic epidermal tissue, with morphant cells outlined in red. Scale bar = 10μm (d) Confocal section (x-y) of mosaic embryo in which membrane-RFP (memRFP, red) mRNA was co-injected with Fuz morpholino and processed for Xeel (interlectin-2) antibody (green). Cell expressing a high level memRFP (right) lacks apical Xeel antibody staining comparable to the neighboring cell (right). (d′) Confocal projection (x-z) of [d] illustrates the loss of apical Xeel staining (green) in the Fuz morphant cell correlated with apical memRFP expression (right). Scale bars in D, d′ = 3μm (e) TEM of section of control Xenopus epidermis shows empty and mucus granule-containing vesicles docked at the apical surface, whereas Fuz morphants (f) display a defect of vesicle fusion with the plasma membrane release, as illustrated by large membrane protrusions (green arrows in [b, f]). Additionally, frequent homotypic vesicle fusion events were observed in secretory cells of Fuz morphants (red arrow). Scale bar in E = 500nm; Scale bar in F= 100nm. Image published in: Gray RS et al. (2009) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |