Xenbase Image ID: 136262

Figure 4. (A)Tubulin lifetimes are the same in unperturbed spindles (control, green symbols) and when microtubule translocation is blocked with FCPT (+FCPT, blue symbols). Best fits to Equation 1 are displayed without FCPT (black line, τ = 64 ± 6 s) and with FCPT (red line, τ = 71 ± 3 s). (B) Increasing concentration of MCAK, a microtubule depolymerizer, results in decreasing tubulin lifetimes. Spindles were assembled in extract with MCAK reduced to 50% native levels (green symbols and black line best fit to Equation 1, τ = 97 ± 11 s) or 200% native levels (blue symbols and red best fit, τ = 47 ± 8 s). Image published in: Needleman DJ et al. (2010) Image downloaded from an Open Access article in PubMed Central. © 2010 by The American Society for Cell Biology Larger Image Printer Friendly View

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