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FIGURE 1:. The purification matrix. (A) The domain structure of S. cerevisiae Stu2 (YLR045C) protein. TOG1 spans residues 8–280, and TOG2 spans residues 326–550. (B) GST-TOG fusion constructs used in this study. (C) Expression and purification of the GST-TOG1/2 fusion protein after overexpression in E. coli. Lanes: Total cell extract before induction (UI) and after induction (I) and the glutathione elute (E). Image published in: Widlund PO et al. (2012) © 2012 Widlund et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |