Xenbase Image ID: 136455

FIGURE 5:. Polymerization competence of tubulins. Electron micrographs of microtubules polymerized from C. elegans (A) and X. laevis (B) tubulin. Tubulins were polymerized in GTP and glycerol, stabilized with Taxol, and then deposited on formvar-coated grids and negatively stained. (C) Human tubulin was polymerized in the presence of GTP and glycerol and the monomer and polymer pools separated by sedimentation over glycerol cushions. Left four lanes, serial dilutions of the total assembly reaction; middle lane, supernatant (Sup); right lane, pellet. Image published in: Widlund PO et al. (2012) © 2012 Widlund et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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