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FIGURE 3:. DNA compaction in dH1, dMock, and dH1+H1. A. Averages of DNA extension decreased as the holding force was reduced progressively from 5 to ∼0.6 pN. The depleted extract (dH1, red square) and dH1 extract plus H1 (dH1+H1, black triangle) were both diluted 20 times (1:20). Each force step took 1 min. At each force, the compaction ratio for dH1 extract was smaller than for dH1+H1 extract. Each experimental data point, presented as the mean value, was obtained from ∼15 measurements. (B) Smoothed time series of DNA compaction in dH1, dMock, and dH1+H1 extracts diluted four times (1:4). The force was initially held at 5 pN for 5 min and then reduced to 2 pN to allow chromatin assembly. The dH1 had slower assembly rates and longer end length than the dMock and dH1+H1. After 10 min, the force was increased to 5 pN. The extension of dH1 increased faster than for dMock and dH1+H1. Each data point is presented as an average and standard error obtained from three or four separate experiments. Image published in: Xiao B et al. (2012) © 2012 Xiao et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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