Xenbase Image ID: 136641

Figure 2. Lpd directly interacts with the SH3 domain of Abi. (A) Pull-down of Lpd from NIH/3T3 cell lysate using the GST-Abi-SH3 domain or GST as control. (B–D) Far Western overlay on different GST-Lpd truncation mutants (B) or GST control using purified (C) MBP-Abi1full-length or (D) MBP-Abi1ΔSH3 was detected with anti-MBP antibodies. Three independent experiments were performed. (E) Far-Western overlay with MBP-Abi1full-length on a peptide array covering the C terminus of Lpd with 12-mer peptides overlapping each other by three amino acids was detected with anti-MBP antibodies. (F) Table shows Abi SH3 domain–binding motifs in the Lpd sequence. The two GST-Lpd fragments highlighted in red correspond to the most strongly interacting Lpd fragments in the Far-Western experiment in C. The amino acid residues highlighted in yellow correspond to the core residues required for class II SH3 domain binding. Image published in: Law AL et al. (2013) © 2013 Law et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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