XB-IMG-136643
Xenbase Image ID: 136643
|
Figure 4. Lpd regulates cell spreading. (A and B) Western blot of cell lysates of Lpd WT and Lpd KO MEFs using anti-Lpd (A) or Scar/WAVE1 (B). Loading control: anti-HSC70. (C and D) F-actin staining (phalloidin) in Lpd WT (C) and Lpd KO MEFs (D). Arrows in C indicate the presence of lamellipodia in Lpd WT MEFs. Arrowheads in D indicate the absence of lamellipodia. (E and F) F-actin staining (phalloidin) determines the area of Lpd WT (E) and Lpd KO MEFs (F) after 60 min of spreading on fibronectin. (G) Quantification of the spreading area of MEFs from E and F. Values are mean ± SEM (error bars) of 131 (KO) or 155 (WT) cells. Unpaired, two-tailed t test: ****, P ≤ 0.0001. Bars, 25 µm. Image published in: Law AL et al. (2013) © 2013 Law et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |