XB-IMG-136644
Xenbase Image ID: 136644
|
Figure 5. Lpd regulates cell migration via Abi and the Scar/WAVE complex. (A and B) Quantification of velocity (A) and persistence (B) of randomly migrating Lpd WT or KO MEFs. Mean population speed and persistence (dt = 2, TR = 4; see Materials and methods for calculation). Results are mean ± SEM (error bars), with three independent experiments. ****, P ≤ 0.0001, unpaired t test. (C and D) A confluent layer of WT or KO Lpd MEFs was scratched, and the area of the scratch measured at 0 and 24 h. Bar, 500 µm. Area closure is shown as the percentage of WT cells. (D) Results are mean ± SEM, with four independent experiments. ***, P ≤ 0.001, unpaired t test. See also Fig. S3 and Videos 1 and 2. (E and F) Lpd overexpression increases cell migration speed via Abi and Scar/WAVE. MDA-MB231 breast cancer cells, stably expressing Nap1-specific (Nap1 shRNA 1 or 2) or scrambled control shRNA were transiently transfected with GFP-Lpd or GFP as control (E) or GFP-Lpd, GFP-LpdEVMut, GFP-LpdAbiMut, GFP-LpdEV+AbiMut, or GFP as control (F). A confluent cell layer was scratched and the area of the scratch was measured at 0 and 24 h. Area closure is shown as percentage increase over GFP cells. Results are mean ± SEM (error bars), from three independent experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns, not significant; one-way ANOVA was used. (E) Tukey’s test. (F) Newman-Keuls method. Image published in: Law AL et al. (2013) © 2013 Law et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |