XB-IMG-136646
Xenbase Image ID: 136646
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Figure 7. Lpd regulates NC migration via Abi. (A) In situ hybridization for Twist (Hopwood et al., 1989; migratory NC marker) in control embryos or embryos injected with Lpd mRNA, Lpd, or Abi MOs (Lpd MO or Abi MO) or the dominant-negatives Lpd N1, Lpd N6, and Abi1ΔSH3. Black arrowheads, normally migrating NC streams. Red arrowheads, streams with impaired migration. Blue asterisks, the eye. (B) Summary of phenotypes. Lpd overexpression has no effect on overall NC cell migration. Lpd MO, Abi MO, and the dominant-negatives Lpd N1, Lpd N6, and Abi1ΔSH3 all impair NC cell migration. (C) Percentages of embryos with normal migration along the dorso-ventral axis (nctl = 26, nLpdRNA = 41, nLpdMO = 37, nLpdN1 = 26, nLpdN6 = 49, nAbiMO = 16, and nAbi1ΔSH3 = 24). (D) Mean distance of migration along the dorso-ventral axis as a percentage compared with control embryos (nctl = 12, nLpdRNA = 14, nLpdMO = 12, nLpdN1 = 14, nLpdN6 = 14, nAbiMO = 12, nAbi1ΔSH3 = 12). One-way ANOVA and Dunnett’s test were used. ***, P < 0.001. (E) In situ hybridization for Twist in Lpd or Abi (Lpd MO or Abi MO), Lpd mRNA and Lpd MO, or Abi mRNA and Abi MO or control MO injected embryos. (F) Mean distance of migration along the dorso-ventral axis compared with control MO embryos (nctl MO = 13, nLpdMO = 14, nLpdMO = 26, nLpdMO+LpdmRNA = 20, nAbiMO = 19, nAbiMO+AbimRNA = 26). One-way ANOVA and Dunnett’s test were used. **, P < 0.01; ns, nonsignificant. (G–I) Lpd and Abi function cell-autonomously in NC migration. (G) Schematic diagram of graft experiment in H. (H) NC from MO-treated embryos or WT embryos was grafted into WT or MO-treated embryos, and NC migration was analyzed. (I) Quantification of NC migration phenotypes from H. Numbers (embryos with inhibition of NC migration/total): nctl>ctl = 0/4 (0%), nLpdMO>ctl = 4/6 (66%), nctl>LpdMO = ¼ (25%), nAbiMO>ctl = 4/5 (80%), nctl>AbiMO = 0/5 (0%). Bars, 150 µm. Image published in: Law AL et al. (2013) © 2013 Law et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |