Xenbase Image ID: 136647

Figure 8. Lpd regulates lamellipodia protrusion and cell migration via Abi in Xenopus NC cells. (A) Localization of Lpd-GFP in Xenopus NC cells cultured on fibronectin. Actin filaments are stained with TRITC-Phalloidin. (B) Cells expressing nuclear mCherry (pseudocolored magenta by thresholding) and membrane GFP (pseudocolored green by thresholding) were used to analyse cell protrusions (pseudocolored red). Cell protrusions were defined as the area of protrusion that extends beyond the cell body. Cell protrusions are shown for control cells and cells injected with Lpd mRNA, Lpd N6 or co-injected with Lpd mRNA and Abi-Δ-SH3. Note that Lpd overexpression leads to enlarged protrusions. This effect is abolished by co-injection with Abi-Δ-SH3.. (C–E) Area of cell protrusions expressed as a proportion of normal protrusions (graph in D: nctl = 58, nLpd800pg = 33, nAbi1ΔSH3 = 24, nLpd+Abi1ΔSH3 = 17 [one-way ANOVA and Dunnett’s test; ***, P < 0.001]; graph in E: nctl = 154, nLpd400pg = 148, nLpd800pg = 157, nLpdN6 = 121 [one-way ANOVA and Tukey’s test; ***, P < 0.001]). (F–J) Tracks of NC cells cultured on fibronectin (a subset of the analyzed tracks are shown). (K and L) Velocity and persistence from tracks performed on all conditions (nctl = 543, nLpdMO = 240, nLpdmRNA = 297, nAbiMO = 180, nLpd+AbiMO = 290). One-way ANOVA and Dunnett’s test were used. *, P < 0.05; ***, P < 0.001. Error bars indicate SEM. See also Fig. S5 and Videos 3–6. Image published in: Law AL et al. (2013) © 2013 Law et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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