XB-IMG-136648
Xenbase Image ID: 136648
|
Figure 9. pico regulates Drosophila border cell migration via SCAR. (A) GST-Pico, the fly orthologue of Lpd, pulled down all Myc-tagged components of the Scar/WAVE complex. Myc-HSPC300 is not shown. (B) pico knockdown or overexpression, or SCAR RNAi under the control of slbo-GAL4 abrogate migration at stage 9. (B, top) Representative images of WT and defective egg chambers. Green, GFP-labeled border cells; red, DNA; blue, F-actin. The box and whiskers plot shows mean border cell position: distance of the cluster relative to the most anterior position of the overlying follicle cells (broken lines). Top and bottom box: 75th and 25th quartile; whiskers indicate minimum and maximum. One-way ANOVA and Dunnett’s test were used. ***, P < 0.001; n = 50. (C) pico overexpression abrogates migration at stage 10A, and this is ameliorated by SCAR RNAi. Histogram summarizes migration defects in the indicated genotypes. Migration was calculated as a percentage of the distance traveled to the oocyte/nurse cell boundary (broken lines in top panels). For each genotype, n = 50 egg chambers. Image published in: Law AL et al. (2013) © 2013 Law et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |