XB-IMG-136656
Xenbase Image ID: 136656
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Figure 2. GFP-IFT20 accumulates in axonemes of Rsg1 KD MCCs. (a) High-magnification confocal image of axonemes from a control MCC expressing GFP-IFT20. The orange box indicates the region shown in a?. See also Additional file 1: Movie 1. (a?) A series of stills from Additional file 1: Movie 1. Yellow dashed lines outline the axoneme and distal is to the right. Pink and blue arrowheads indicate an anterograde train and a retrograde train, respectively. (b) High-magnification confocal image of axonemes from an Rsg1 KD MCC expressing GFP-IFT20. Orange box represents the region shown in b?. See also Additional file 2: Movie 2. (b?) A series of stills from Additional file 2: Movie 2. Yellow dashed lines outline the axoneme and distal is to the right. No IFT trains are visible during the course of the movie. (c?c?) A single confocal slice of an control MCC co-expressing membraneRFP and GFP-IFT20. (d?d?). A single confocal slice of an Rsg1 KD MCC co-expressing membraneRFP and GFP-IFT20. (e?e?) A single confocal slice of an Rsg1 KD2 (second-site morpholino) MCC co-expressing membrane-RFP and GFP-IFT20. Yellow arrowheads indicate large GFP-IFT20 accumulations in distal axonemes. (f) Quantification of mean GFP-IFT20 signal along control and Rsg1 KD axonemes, as normalized to membrane-RFP intensity along the same length. Note the increase in normalized IFT20 signal in Rsg1 KD axonemes (Ctl: 0.39 ? 0.17, n = 304 axonemes, 36 cells, 7 embryos vs. Rsg1 KD: 0.75 ? 0.45, n = 223 axonemes, 31 cells, 6 embryos; ***P <0.0001). (g) There is no significant difference in axonemal average membrane-RFP signal as normalized to cortical membrane-RFP signal from the same cell, between control and Rsg1 KD conditions (Ctl: 1.04 ? 0.33, n = 28 cells, 6 embryos vs. Rsg1 KD: 1.21 ? 0.36, n = 21 cells, 6 embryos; P = 0.2607). Scale bars in a?b? indicate 3 ?m. Scale bars in c-e? indicate 5 ?m. Time stamps in a? and b? are relative to the first frame of Additional file 1: Movie 1 and Additional file 2: Movie 2, respectively. Image published in: Brooks ER and Wallingford JB (2013) Copyright © 2013 Brooks and Wallingford; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |