XB-IMG-136685
Xenbase Image ID: 136685
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Figure 2. GALNT11 glycosylation of NOTCH1 derived peptides and effects on ADAM-mediated cleavagea) Depiction of NOTCH1 with MALDI-TOF spectra demonstrating O-GalNAc glycosylation of peptides derived from EGF6 (blue), EGF36 (magenta) and the juxtamembrane region (green). Yellow squares: O-GalNAc, Red triangles: O-Fuc. TM: transmembrane domain; NICD: Notch intracellular domain; # deammoniated peptide; & sodium adducts. b) MALDI-TOF time-course analysis of in vitro cleavage of the juxtamembrane peptide and glycopeptide by ADAM17. Scissors: Cleavage site. *nonspecific N-terminal degradation of two amino acids. c–d) HPLC analysis of the cleavage reactions with c) naked peptide and d) glycopeptide after 2 hrs (percentages above the peaks) with p<0.05 (two-tailed t-test). Image published in: Boskovski MT et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |