Xenbase Image ID: 136686

Figure 3. Galnt11/Notch signaling switches cilia between motile and immotile typesa) Wildtype LRO explants expressing arl13b-mCherry reveal distinct populations of immotile and motile cilia. Cyan box highlights a single motile cilium, with a corresponding kymograph analyzing beating frequency. Magenta box highlights immotile cilium and kymograph. b) Analysis of multiple vehicle LROs reveals a specific geography for cilia types: immotile cilia are enriched along the fringes, while motile cilia are enriched in the center. c) Schematic of a wildtype LRO A: anterior, P: posterior, L: left, R: right. d) nicd overexpressants exhibit a decrease in motile cilia in the center of the LRO. notch1 (e) and galnt11 (f) morphants display a decrease in immotile cilia along the LRO periphery. (g–h) Quantification of motile cilia distribution in total LROs (g), and in the center and along the periphery of the LRO (h). Bars depict means and error bars are standard error. b,d,e,f) n=8. g,h) vehicle n=17, nicd RNA n=12, notch1 MO n=12, galnt11 MO n=15. Additional details are in Methods. Image published in: Boskovski MT et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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