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Fig. 5. Gene activity profiles showing successive waves. Each plot shows the number of genes with onset times at each sampling point in the various time series, showing the data for different fold change thresholds (see text). Positive vertical axes represent onset of rising transcript levels, negative axes represent onset of falling levels. All data are plotted against actual sample times, except the 2× Series 3 in A, which is corrected for development rate (Materials and Methods). MBT (pink bar) gives consistent developmental time scale. (A) PolyA+ Series 1 and 2. Also shown is time-adjusted Series 3 data using a more sensitive end-of-series 2× threshold (Materials and Methods) to show detection of the third wave in this developmentally shorter time series. (B) PolyA+ Series 3. (C) PolyA+ Series 3 analysed at one-hour intervals. (D) Ribo-Zero Series 3. (E,F) Activity profiles using 10× threshold, organised by gene type: TF, transcription factor; SIG, signalling molecules and receptors; other, genes not classified as TF or SIG. Stage diagrams reproduced from Nieuwkoop and Faber (Nieuwkoop and Faber, 1994) with permission of Garland Science/Taylor & Francis, LLC. Image published in: Collart C et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |