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Fig. 8. Variable transcriptional deficit at MBT in embryos treated with cordycepin. (A) Confirmation of inhibition of polyadenylation: shortened polyA+ tail lengths in cordycepin-treated embryos for the polyadenylated maternal genes VegT, Fam46c and Ccnb3. Lanes: ‘co’, long and variable polyA+ tail in controls; ‘cordycepin’, consistently short polyA+ tail in treated embryos; ‘restriction digest’, confirmatory restriction digest of a gene-specific PCR fragment (Materials and Methods). (B-D) Transcriptional activation of genes in cordycepin-treated versus control embryos by NanoString nCounter (red/blue) or qPCR (green/black). (B) Transcription unaffected by cordycepin treatment. (C) Substantially reduced transcription levels after cordycepin treatment. (D) Loss of primary transcripts for Mix1 shown by intron qPCR confirms loss of transcription, not degradation of deadenylated transcripts. Image published in: Collart C et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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