Xenbase Image ID: 136910

Figure 4. Duration of actomyosin contractions depends on temperature.(A) Sequential frames from a representative time-lapse sequence collected from the basal cortex of an animal cap ectoderm explant cultured on fibronectin-coated glass substrate. F-actin dynamics are revealed in cells expressing the actin-binding domain from moesin coupled to EGFP (moe-GFP) (left column). This sample collected at 16°C. (A′) Schematic of frames matching those in (A) highlighting the cell outline (dotted line) and hexagonal regions of the cell cortex identified as “F-actin contractions.” Regions are categorized as contractions when their integrated intensities are 50% greater than the mean intensity of the basal cell cortex. (B) Duration of individual F-actin contractions across the three temperature regimes. (C) Frequency distribution of the duration F-actin contractions at three temperatures. Note abundant short duration contractions at the low temperature regime. Image published in: von Dassow M et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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