Xenbase Image ID: 137086

Figure 1. Identification and characterization of USP15 as an interactor of SMAD6. (a) Coomassie stained gel image showing anti-GFP-IPs from HEK293 extracts expressing GFP-SMAD6. The interacting proteins were excised as 2 mm gel pieces, digested with trypsin and identified by mass spectrometry. The location where USP11 and USP15 were identified is indicated. All protein interactors of GFP-SMAD6 identified by mass spectrometry are indicated in the right panel. Protein interactors of GFP control were removed from the list of GFP-SMAD6 interactors. (b) HEK293 cells were transfected transiently with FLAG-SMADs. Extract inputs were resolved by SDS-PAGE and subjected to immunoblotting (IB) with anti-USP11, anti-USP15 and anti-FLAG antibodies as indicated. (c) FLAG-IPs were subjected to immunoblotting with anti-USP11 and anti-FLAG antibodies. (d) Endogenous pre-immune IgG or anti-USP15 IPs were subjected to immunoblotting with anti-FLAG and anti-USP15 antibodies as indicated. Image published in: Herhaus L et al. (2014) . This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page