Xenbase Image ID: 137210

FIGURE 5:. Structural changes induced by RNA editing lead to reduced processing of pri-miRNA-142. Constructs mimicking wild-type, preedited, and constructs carrying compensatory mutations that revert secondary structure changes induced by RNA editing were injected into eggs, and their processing was monitored. (A–D) Constructs used for injection. (A) Wild-type pri-miRNA-142, (B) pri-miRNA-142 preedited at positions +4 and +5, (C) editing incompetent pri-miRNA-142, and (D) compensatory mutation closing the loop induced upon editing. Red nucleotides mark adenosines reported to be edited (Yang et al., 2006). Blue nucleotides mark the position of introduced mutations. (E) Processing of pri-miRNA-142 variants in Xenopus eggs shows that processing is strongly inhibited in the preedited pri-miRNA-142. RNAs were reextracted 30 min after injection from individual microinjected eggs and separated on denaturing polyacrylamide gels. Processing can be restored by mutations that prevent editing or a compensatory mutation that restores proper folding. (F) Quantification of processing levels (ratio of pre-miRNA to pri-miRNAs) shows a strong reduction in processing in preedited pri-miRNA 142. Processing ratio of wild-type pri-miRNA-142 to pre-miRNA-142 was set to 1, and all other processing ratios were normalized to this. For quantification, different exposures were chosen to prevent saturated pixels in the pri-miRNA region and obtain sharp bands in this region. Image published in: Muggenhumer D et al. (2014) © 2014 Muggenhumer, Vesely, et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page