XB-IMG-137233
Xenbase Image ID: 137233
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Figure 5. The influence of miR-155 on ribosomes, mRNA and tails in the early zebrafish
embryoa, Relationship between changes in ribosome protected
fragments (RPFs) and changes in mRNA levels after injecting miR-155. Changes
observed between miRNA- and mock-injected embryos are plotted at the indicated
stages for predicted miR-155 target genes (red, genes with ≥1 miR-155
site in their 3′ UTR) and control genes (gray, genes that have no
miR-155 site yet resemble the predicted targets with respect to 3′ UTR
length). To ensure that differences observed between 4 and 6 hpf were not the
result of examining different genes, only site-containing genes and no-site
control genes detected at both 4 and 6 hpf are shown for these stages. Lines
indicate mean changes for the respective gene sets, with statistically
significant differences between the sets indicated (*, P
≤0.05; **, P <10−4,
one-tailed Kolmogorov–Smirnov test). Because injected miRNAs partially
inhibited miR-430–mediated repression, genes with miR-430 sites were not
considered. Data were normalized to the median changes observed for the
controls. b, Relationship between RPF changes and mean tail-length
changes after injecting miR-155. Tail-lengths were determined using PAL-seq,
otherwise as in a. c, A developmental switch in the
dominant mode of miRNA–mediated repression. The schematic (left) depicts
the components of the bar graphs, showing how the RPF changes comprise both mRNA
and TE changes. The compound bar graphs show the fraction of repression
attributed to mRNA degradation (blue) and TE (green) for the indicated stage,
depicting the overall impact of miR-155 (center; plotting results from
a and b for genes with sites) and miR-132 (right,
plotting results from Extended
Data Fig. 8b for genes with sites). Slight,
statistically insignificant, increases in mRNA for predicted targets resulted in
blue bars extending above the axis. For samples from stages in which tail length
and TE are coupled, a bracket adjacent to the compound bar indicates the
fraction of repression attributable to shortened tails. Significant changes for
each component are indicated with asterisks of the corresponding color (*,
P ≤0.05; **, P
<10−4, one-tailed Kolmogorov–Smirnov
test). Image published in: Subtelny AO et al. (2014) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |