XB-IMG-137342
Xenbase Image ID: 137342
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Extended Data Figure 3. miR-34/449 miRNAs are enriched in airway MCCs a. Most miR-34/449 miRNAs are enriched in tissues with motile cilia. Using real time PCR, the expression of miR-34a, miR-34c, and miR-449c were measured in multiple tissues from newborn, P10, P20, and adult wild-type mice. Both miR-34c and miR-449c are exclusively expressed in tissues with motile cilia, while miR-34a exhibits a broader expression pattern. n=3. b. The real time PCR assay for each miR-34/449 miRNA specifically detects the corresponding miRNA. The specificity of each miRNA real time PCR assay was validated using testis RNA from wild-type (WT), mir-34a-/-, mir-34b/34c-/-, mir-449-/-, and TKO mice at postnatal day 35. The miR-449a assay shows a slight cross reaction with homologous miRNAs. n=3. c.
In situ hybridization of each miR-34/449 miRNA exhibits specific detection. No measurable miR-34/449 in situ signal is detected in TKO lung sections at postnatal day 25. n=2. d.
miR-34/449 miRNAs are enriched in tracheal MCCs. In situ hybridization analyses demonstrate that miR-34c and miR-449c are specifically expressed in the tracheal MCCs, while miR-34a is expressed in both tracheal MCCs and the surrounding cell types. n=2. e. miR-34/449 TKO mice do not exhibit significant alterations in Foxj1 expression. Quantification of Foxj1 positive cells (left, n=3) and foxj1 mRNA (right, n=4) was performed for well controlled wild-type, DKO and TKO tracheas, using immunofluorescence and real time PCR, respectively. Paired t-test, ns: P > 0.05. f. mir-34a-/-; mir-449-/- DKO tracheal epithelia are morphologically indistinguishable from wild-type controls in scanning electron microscopy (SEM) analyses. n=3. All error bars represent s.e.m. Image published in: Song R et al. (2014) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |