Xenbase Image ID: 137344

Extended Data Figure 5. Major basal body structural components are intact in miR-34/449 TKO MCCs revealed by transmission electron microscopyApically docked (a) and undocked (b) basal bodies in miR-34/449 TKO MCCs have intact structural components. Basal body transition fibers (top), basal feet (middle) and striated rootlets (bottom) have comparable morphology among WT, DKO and TKO MCCs. Top panel: arrow, a representative transverse view of transition fibers. Middle panel: arrow, a representative transverse view of nine microtubule triplets with basal feet; arrowhead, a representative transverse view collected from a different height of a basal body, containing nine microtubule triplets without basal feet. Bottom panel: arrow, the longitudinal view of basal feet; arrowhead, the striated rootlet structure. c. Directionality of basal bodies (top) and axonemes (middle) is moderately affected in miR-34/449 TKO MCCs. Top panel: arrows point to the directions indicated by basal foot. Middle panel: red lines connecting the central pair of axonemes indicate the rotational polarity of each ciliary axoneme. Bottom panel: the angles of the axoneme directionality were statistically analyzed as bidirectional circular data. The average angel was set from 0° to 180° axis. miR-34/449 TKO ciliary axonemes have moderately un-coordinated directionality compared to WT and DKO controls. d. miR-34/449 TKO axonemes exhibit intact structures, including nine outer microtubule doublets, two central microtubule singlets, and dynein arms. n=3. Image published in: Song R et al. (2014) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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