Xenbase Image ID: 137348

Extended Data Figure 9. Gain and loss of cp110 affects MCC basal bodies, but not the subapical Actin meshworka. cp110 overexpression phenocopies miR-34/449 knockdown. Centrin4-RFP enrichment is strongly reduced in cp110Δ3′UTR overexpressing MCCs. It is noteworthy, that while ciliation and incorporation of Centrin4 are strongly affected in cp110Δ3′UTR injected embryos, formation of the subapical Actin meshwork appears largely unaffected. Together with the lack of cp110 knockdown to rescue the subapical Actin meshwork in miR-34/449 morphants, these data indicate an additional effect of miR-34/449 miRNAs on Actin formation/organization, which is cp110 independent. Cilia: Ac-α-tub, basal bodies: Centrin4-RFP, Actin: phalloidin-488. Embryos/cells analyzed: Uninjected (4/7), cp110Δ3′URT (6/10). b. The cellular basis for ciliation defects in cp110 morphants is likely due to the atypical failure of basal bodies to separate from each other, thus they appear to be aggreageted in clusters of cp110-deficient MCCs. Nevertheless, Centrin4 incorporation or apical localization of basal bodies is not affected in cp110 morphants. Basal bodies: Centrin4-RFP, Actin: phalloidin-488. Embryos/cells analyzed: Uninjected (2/3), cp110 MO (5/8). Embryos were derived from at least two females and independent fertilizations per Xenopus experiment. Larger Image Printer Friendly View

Return to previous page