XB-IMG-137435
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Figure 5. Genome-wide Oocyte Linker Histone B4 Binding to the Chromatin of Transplanted Nuclei(A) The binding of B4 was analyzed in the ±20 kB region surrounding the TSS of all mouse genes. The graph shows the normalized read count for B4 (top [i]) and H3 (bottom [ii]) across this region. The longitudinal axis shows reads per billion per gene in each bin.(B) Distribution of B4 peaks in different genomic regions. B4/H3 peaks per megabase were calculated across different genomic regions. The dotted line shows the whole genome average value. Statistical significance was evaluated against peaks in the whole genome. p values were calculated using t test. ∗∗∗p < 0.0001; ∗∗p < 0.001; and ∗p < 0.01.(C) Same as in (A) but for the genes that are expressed (green) and not expressed (red) after NT (as determined by RNA-seq analysis in Figure 1B).(D) HHB overexpression impairs activation of reprogrammed genes that were identified by RNA-seq analysis. Gene reactivation in NT oocytes was judged by RT-qPCR analysis. Data are represented as mean ± SEM; n = 4. p values were calculated using ANOVA; ∗p < 0.01.See also Figure S5. Image published in: Jullien J et al. (2014) Image downloaded from an Open Access article in PubMed Central. © 2014 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society Larger Image Printer Friendly View |