Xenbase Image ID: 137570

Fig. 5. Knockdown of BAF60a or BAF60b restores myogenesis after miRNA inhibition. (A) Somites injected with AM133 were electroporated with FITC-labeled control-MO or BAF60a-MO, as indicated. In situ hybridization for myogenin transcripts (purple) and detection of FITC-MO (red) shows that myogenic differentiation is inhibited in the presence of AM133 plus control-MO (top row, arrows and arrowheads); whole-mount and section are shown. Co-electroporation of BAF60a-MO with AM133 rescued the expression of myogenin (bottom row). (B) Somites injected with AM1 plus AM206 were electroporated with FITC-labeled control-MO or BAF60b-MO, as indicated. In situ hybridization for myogenin transcripts (purple) and detection of FITC-MO (red) shows that myogenic differentiation is inhibited in the presence of AM1 and AM206 plus control-MO (top row, arrows and arrowheads). Myogenin expression is rescued when BAF60b-MO is co-electroporated with AM1 and AM206 (bottom row, arrows and arrowheads). Scale bar: 50 µm. (C) Summary of phenotypes observed after AM/MO injections. my, myotome; nt, neural tube; nc, notochord. Image published in: Goljanek-Whysall K et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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