Xenbase Image ID: 137602

Figure 12. FoxA4MO reproduced the effects on anterior neural markers in embryos in which mesoderm induction was blocked with Cer-S.(A–D) Expression of foxA4 in sibling controls (A, C) or in embryos injected with Cer-S mRNA (B, D), analysed at stage 9 (A, B) or at stage 11 (C, D). After blocking mesoderm induction, foxa4 expression persisted in the BCNE (B) (100%, n = 15) but was suppressed from the axial mesoderm (D) (100%, n = 7). (E, F) MyoD expression at neural plate stage in a sibling control (E) and in an embryo injected with Cer-S. Mesoderm was suppressed in 100% of the injected embryos analyzed with myoD (100%, n = 8). (G–I) Expression of Xanf1 and en2 at neural plate stage in a sibling control (G), and in embryos in which mesoderm induction was blocked with Cer-S and were injected at the 1-cell stage with either 20 ng of CtrlMO (H) or 20 ng of FoxA4MO (I). Embryos injected with CtrlMO + Cer-S showed expression of Xanf1 (yellow arrow) and en2 (black arrow) (H, 100%, n = 8). In embryos injected with FoxA4MO + Cer-S, Xanf1 was expanded and up-regulated (yellow arrow) and en2 was down-regulated (black arrow) (I, 100%, n = 10) in comparison to embryos injected with CtrlMO + Cer-S (H). (J–L) Expression of otx2 at neural plate stage in a sibling control (J), and in embryos in which mesoderm induction was blocked with Cer-S and were injected at the 1-cell stage with either 20 ng of Ctrl MO (K) or 20 ng of FoxA4MO (L). Embryos injected with CtrlMO + Cer-S showed expression of otx2 (K, 100%, n = 4). In embryos injected with FoxA4MO + Cer-S, otx2 was down-regulated (L, 100%, n = 20) in comparison to embryos injected with CtrlMO + Cer-S (K). Image published in: Murgan S et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Throughout NF stage 9 dorsal marginal zone foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Throughout NF stage 11 dorsal marginal zone upper blastopore lip involuted dorsal mesoderm mesoderm myod1.L mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 presomitic mesoderm rostral presomitic mesoderm caudal presomitic mesoderm en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 15 presumptive midbrain-hindbrain boundary hesx1.L anf2, hesx1-a, hesx1-b, rpx, Xanf, Xanf-1, XANF-2, Xanf1, Xanf2 X. laevis Throughout NF stage 15 anterior placodal area anterior neural ridge neural plate pre-chordal neural plate otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 15 eye primordium anterior placodal area anterior neural ridge neural plate pre-chordal neural plate neural plate border Image source: Published Larger Image Printer Friendly View

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