Xenbase Image ID: 137603

Figure 13. Depletion of FoxA4 from the ectoderm expands Xanf1 and suppresses en2 in the anterior neural plate.(A) Embryos were injected at the 1-cell stage with 20 ng of FoxA4MO +20 ng of DOG (green) or were left uninjected (grey). At stage 9, ectodermal explants were excised and the following recombinants were obtained: in Control recombinants, the ectodermal explant of a recipient uninjected embryo was replaced with the ectodermal explant from an uninjected donor. In FoxA4MO ect/Control mes recombinant, the ectoderm was provided by a FoxA4MO-injected donor (green), while the mesoderm derived from an uninjected sibling recipient (grey). In Control ect/FoxA4MO mes recombinant, the ectoderm was provided by an uninjected sibling donor (grey), while the mesoderm derived from a FoxA4MO-injected recipient (green). (B–E′) Analysis of Xanf1 and en2 when uninjected sibling controls (B) reached neurula stage. (C,C′) Control recombinant. (D–D′) FoxA4MO ect/Control mes recombinant. (E–E′) Control ect/FoxA4MO mes recombinant. (D′ and E′) DOG fluorescence of the images shown in D′, E′, respectively. Image published in: Murgan S et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 15 presumptive midbrain-hindbrain boundary hesx1.L anf2, hesx1-a, hesx1-b, rpx, Xanf, Xanf-1, XANF-2, Xanf1, Xanf2 X. laevis Throughout NF stage 15 anterior placodal area neural plate pre-chordal neural plate neural plate border Image source: Published Larger Image Printer Friendly View

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