Xenbase Image ID: 137791

Figure 4. ATP does not inhibit NFA‐activated Slo2.1 channels heterologously expressed in Xenopus oocytes. (A) NFA activates WT and K1031A Slo2.1 channel currents measured by TEVC. Under control conditions, only endogenous oocyte currents were measurable. After addition of 1 mmol/L NFA, ISlo2.1 was activated to a similar extent in oocytes expressing either WT Slo2.1 channel (left panel) or K1031A mutant Slo2.1 channels (right panel). Upper inset depicts voltage‐clamp protocol. (B) Average I–V relationships for WT (□,■) and K1031A (●,○) ISlo2.1 recorded before and after treatment with 1 mmol/L NFA (n = 17 for each channel type). Oocytes were recorded after 2 days of injection with 1 ng WT or K1031A Slo2.1 cRNA. NFA‐activated currents were larger than control currents (P < 0.001; two‐way ANOVA), but not different between WT and K1031A oocytes. Image published in: Garg P and Sanguinetti MC (2014) © 2014 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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