XB-IMG-137860
Xenbase Image ID: 137860
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Figure 2. TRα TALEN founder developmental phenotypes. A, mCherry mRNA was coinjected with TRα TALEN mRNA into a single blastomere of two-cell stage embryos. Ventral view of the injected tadpole shows mCherry expression only on the left side of its body. B and C, Hind limb and skin phenotype are shown. B, Ventral view of the lower abdomen and proximal part of tail shows different developmental progression in the hind limbs between uninjected and injected sides. White arrows point to side views of the hind limb on each side to show the alteration of not just limb length but stage of limb development. C, Skin on the injected side of a NF stage 57 tadpole shows advanced iridophore development (gold skin color, black triangles). Thicker skin obscures the ultimobranchial body visible only on the uninjected side (white triangle). The dotted line delineates the uninjected and injected sides. D, Histogram of hind limb stage difference in TRα TALEN founders. When the stage of hind limb development on the uninjected side was NF stage 49, the difference in stage compared with the injected side was determined and plotted in the histogram. The hind limb on the injected side was always more advanced, typically by 1 to 1.5 stages (n = 42 injected animals from the same clutch). Tadpoles that lacked a stage difference were not quantified. HL, hind limb. Image published in: Choi J et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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