XB-IMG-137866
Xenbase Image ID: 137866
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Fig. 1. fezf2 knockdown leads to defects in forebrain neuronal differentiation. (A) Whole-mount in situ hybridisation for arx in control MO (20/20) or fezf2 MO (15/18) injected Xenopus embryos. Arrowhead indicates the forebrain. (B-J) One blastomere at the 2-cell stage was injected with fezf2 MO and embryos were sectioned at stage 30 transversely across the forebrain, and stained for Sox3 (B,C), MyT1 (E,F) or TUNEL (H,I). FITC staining identifies the injected side (B,E,H). Arrowheads indicate MyT1+ (E,F) or TUNEL+ (H,I) cells. (D,G,J) Statistical analysis of Sox3+ (n=4 embryos), MyT1+ (n=6 embryos) and TUNEL+ (n=4 embryos) cells. All control sides have been normalised to 100%. Error bars represent s.e.m. *P<0.05; ***P<0.001; ns, not significant. Scale bar: 25 µm. Image published in: Zhang S et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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