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Fig. 3. Whole-mount staining of control and RA-treated tadpoles with anti-neurofilament and anti-muscle antibodies. (A-D) Staining with 68 K anti-neurofilament antibody. A is a dorsal and C a side view of a control embryo. B and D represent the dorsal and side views respectively of embryos treated with 0.5 fm RA. For all panels anterior is to the left and posterior to the right. Note the anterior shift of the otic vesicle (OV) in the RAtreated embryos (B and D). In A and B white arrows mark the limits of the hindbrain with the midbrain and spinal cord, and in the RA-treated embryos (B) while the overall length of the hindbrain is similar to controls it has a broadened appearance. In C (controls) the small arrows mark the three exit points of the Vth, Vllth/VIIIth and EXth cranial nerves, and in RA-treated embryos (D) only two exit points are clearly distinguishable as marked by the arrows. Spinal nerves are indicated by solid arrowheads in A and C. (E-G) Staining with anti-muscle antibody 12/ 101. In E, side view of control tadpole (top) and 0.5 ^M RA-treated embryo (bottom). Eye is indicated by black arrowhead. Dorsal views of the same embryos are shown (F, control; G, RA-treated). White arrows (F and G) point to the position of the narrowing of the IVth ventricle (obex) and indicate that the posterior juncture of the hindbrain with the spinal cord occurs at the same position in control and RA-treated embryos, relative to the first somite. Image published in: Papalopulu N et al. (1991) Copyright © 1991. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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