Xenbase Image ID: 137978

Figure 3 Overexpression of the FGFR-VT+ but not the VT− isoform leads to abnormal development inXenopus. FGFR-VT+ and VT− cRNA was prepared and microinjected into fertilized eggs as described under “Experimental Procedures.” Control embryos (Con) were injected with the same volume of diethyl pyrocarbonate-treated H2O. A, embryos were left to develop for 72 h at room temperature until they reached tadpole stage and then scored for normal development (n); the percentage is based on the total number injected. A total of 150 embryos were used for each experiment, and the averages and S.D. values of 14 individual experiments are shown.B, total RNA (five embryos per treatment) was extracted at 24 h after injection and analyzed by RT-PCR (as described in the legend to Fig. 1) for VT+, VT−, and histone H4 (input control) expression levels. The positions of the VT+, VT−, and H4 PCR products are indicated. C, embryo proteins were labeled by injection of [35S]methionine, and after 24 h, total protein was extracted (100 embryos per sample), immunoprecipitated, and analyzed as described under “Experimental Procedures.” The position of FGFR1 protein is indicated. Image published in: Paterno GD et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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