Xenbase Image ID: 137993

FIG. 3. Mapping of the Smad-binding domain in SIP1. Schematic representation of deletion constructs to map the segment required for interaction of SIP1 with the XSmad1 MH2 domain. The original Smad-binding domain (spanning amino acids 315–507; shown in brown) was used as a starting point for the detailed mapping. The right column summarizes interaction data obtained in yeast, as assayed by ability to grow on plates lacking histidine and in the presence of 5 mM 3-amino-triazole and by staining of b-galactosidase. The Smadbinding domain (SBD, shown in green) is a 51-amino acids-long peptide defined as essential for the interaction; its amino acid sequence (aa seq) is shown in the lower part of the figure. Image published in: Verschueren K et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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