Xenbase Image ID: 137994

FIG. 4. In vitro association of SIP1 with the MH2 domains of different Smad proteins. Myc-tagged full-size SIP1 protein was expressed in COS1 cells. Upper panel, equal amounts of the same cell extract were used in lanes 1–6. The 145-kDa SIP1 protein (indicated by the arrow) was efficiently pulled down from this cell lysate using the different GST-Smad fusion proteins (lanes 1 and 4–6; visualization is by Western blotting using anti-Myc antibody) but not by an unrelated GST-fusion protein (GST-CD40; lane 3) and GST (lane 2). GST fusions included the MH2 domains of XSmad1(G418S) (lane 4), mouse Smad5 (lane 5), mouse Smad2 (lane 6), and wild type XSmad1 (lane 1), respectively. Lane 7 provides a negative control with proteins pulled-down by GST-XSmad1 from a cell lysate of mock-transfected cells. Lower panel, estimate of the amount of GST-fusion proteins used in the pull-down experiments by Ponceau S staining of the used blot. Lower amounts of GST-Smad fusion proteins were used in lanes 1 and 4–7. Image published in: Verschueren K et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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