XB-IMG-137996
Xenbase Image ID: 137996
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FIG. 6. Interaction of the DNA-binding
domain of SIP1 (SIP1CZF) with
target sites of different promoters by
gel retardation analysis. A, the fusion
protein GST-SIP1CZF (10 ng) was incubated
with the indicated 32P-labeled
double-stranded oligonucleotides. No
binding was observed with negative control
GST-PLAG1DBD (Control; the Brabinding
site was used here). B, interaction
of SIP1CZF with E2 box sequences. The
experiment was carried out as described
in panel A, but MyoD and MyoD-Mut labeled
probes were used. Competition experiments
were carried out with the oligonucleotides
listed above the lanes. In
the negative control lanes (Control), both
MyoD and MyoD-Mut probes were incubated
with GST-PLAG1DBD, and no competitor
was added. No, no competitor
added. Image published in: Verschueren K et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |