XB-IMG-138001
Xenbase Image ID: 138001
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FIGURE 3. Impaired cardiac development in HIF-1-depleted Xenopus
embryo. A, a representative result of images obtained from embryos uninjected
(control) or injected with hif-1 MO1or hif-1 MO2. Panel a, embryos
without injection possessed a beating heart (white arrowhead) anterior to the
gut. Panels b and c, embryos injected with either hif-1 MO1or hif-1 MO2(40
ng) had either a tissue-free region where the heart should localize or hearts
that did not beat (yellow arrowhead) at stage 42. B, expression of cTnI examined
by in situ hybridization in HIF-1-depleted embryos. The embryos
injected withCMO(40 ng) (a), hif-1 MO1(40 ng) (b), or hif-1 MO2(40 ng) (c)
were analyzed at stage 33/34. Panels show anterior ventral views. Yellow
arrowheads point to the side derived from the blastomere where hif-1 MO
was injected. In hif-1 MO-injected embryos, cTnI was detected in two separate
populations of cardiomyocytes, whereas cTnI was detected at the center
in CMO-injected embryos. Representative eosin-stained cross-sections of
embryos injected with CMO (b), hif-1 MO1 (d), or hif-1 MO2 (f) are shown.
The heart region is indicated by an arrowhead. Image published in: Nagao K et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |