XB-IMG-138004
Xenbase Image ID: 138004
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HIFFIGURE
6. NKX2.5 functions downstream of HIF-1. A, cardia bifida in HIF-
1-depleted embryos was rescued by expression of nkx2.5. Embryos were
injected with hif-1 MO1 (40 ng) and nkx2.5 mRNA (0, 125, and 250 pg). The
expression pattern of cTnI was analyzed by in situ hybridization at stage 33/34
to detect the precise migration of cardiomyocytes. Cardia bifida caused by
depletion of HIF-1 was restored by co-injection of nkx2.5 mRNA. B, depletion
of NKX2.5 results in cardia bifida in Xenopus embryos. CMO-injected embryos
(a), nkx2.5 MO-injected embryos (b), and hif-1 MO1-injected embryos (c)
were examined for cTnI by in situ hybridization at stage 33/34. NKX2.5-depleted
embryos showed a similar staining pattern of cTnI to HIF-1-depleted
embryos. Yellow arrowheads indicate the side derived from the blastomere
where hif-1 MO1 was injected. C, the incidence of cardia bifida in nkx2.5
MO-injected embryos, hif-1 MO-injected embryos, or CMO-injected
embryos. Image published in: Nagao K et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |